Monday, February 27, 2012

Separation of DNA Fragments Using PAGE Method | Help Biotech

Separation of DNA Fragments Using PAGE Method | Help Biotech

This method has the capacity to separate DNA fragments using the size of as small as 10 bp or longer to 1 kb with all the resolution regarding as little as 1 bp. While agarose gel electrophoresis is just capable of distinct DNA fragments while using bigger dimension of which PAGE really does or even in the size selection of 100 nucleotides to help close to 10 - 15 kb.

Wednesday, February 22, 2012

Bacterial DNA Taking out for PCR | Help Biotech

Bacterial DNA Taking out for PCR | Help Biotech

Bacterial DNA taking out for PCR

Some polymerase chain impulse (PCR) applications for instance gene prognosis or perhaps typing, need little purified DNA and may even possibly be performed together with crude bacterial extract. Several approaches are actually described just for this course of action along with a number of them are straightforward and also are comprised purely boiling bacterial cells in water. When it reaches this probability, we would like to reveal the extraction way of bacterial DNA for PCR that works well having Gram-positive bacteria such as staphylococci, enterococci, and also Clostridium difficile.

see more: http://www.bio8.info/2012/02/bacterial-dna-taking-out-for-pcr.html